Polymorphism of Leukocyte and Erythrocyte Antigens in Chronic Kidney Disease Patients in Southern BrazilReport as inadecuate




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We investigated the polymorphism of human leukocyte antigens HLA and Duffy erythrocyte antigens in chronic kidney disease CKD patients in southern Brazil. One hundred and eighty-three CKD patients, over 18 years old, on hemodialysis, were included. HLA-A -B and -DRB1 typing was performed using the LABType®SSO One Lambda, Inc

Duffy phenotypes were determined by gel column agglutination using anti-Fya and anti-Fyb monoclonal anti-sera. The patients- predominant ages ranged between 51 and 70 years 43% and the predominant gender, ethnic group and dialysis period were, respectively, male 62%, white 62% and 1–3 years 40%. The highest and lowest frequencies of Duffy phenotypes were Fya+b+ and Fya−b−, respectively. Nineteen HLA-A, 30 HLA-B and 13 HLA-DRB1 allele groups were identified. The most frequent HLA allele groups were HLA-A*01 -A*02 -A*03 -A*11 -A*24; HLA-B*07 -B*15 -B*35 -B*44 -B*51; HLA-DRB1*03 -DRB1*04 -DRB1*07 -DRB1*11 and -DRB1*13. Statistically significant differences were observed in the Duffy and HLA polymorphisms compared between CKD patients and healthy subjects. The Fya+b− phenotype p<0.0001, OR = 2.56, 95% CI = 1.60–4.07 was the most frequent in the patients p<0.05, and the Fya+b+ phenotype p = 0.0039, OR = 1.71, 95% CI = 1.18–2.51 was the most frequent in the healthy subjects in the same region of Paraná state p<0.05. Regarding HLA, the HLA-B*42 -B*45 -B*51 and -DRB1*03 allele groups were the most frequent in the patients p<0.05, and the HLA-B*44 allele group was the most frequent in the healthy subjects in the same region of Brazil p<0.05. The polymorphism of these two markers among CKD patients in southern Brazil and healthy subjects of other studies, suggests that these markers might be involved with CKD development. Further studies should be undertaken to analyze the markers- influence on CKD and the long-term results from kidney transplantation.



Author: Roger Haruki Yamakawa, Patricia Keiko Saito, Waldir Veríssimo da Silva Junior, Luiz Carlos de Mattos, Sueli Donizete Borelli

Source: http://plos.srce.hr/



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