Label-Free Imaging of Lipid Depositions in C. elegans Using Third-Harmonic Generation MicroscopyReport as inadecuate




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Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, imaging fatty acid distribution and dynamics in vivo, at the cellular or organismal level is challenging. We developed a label-free method for visualizing lipid depositions in vivo, based on third harmonic generation THG microscopy. THG imaging requires a single pulsed-laser light source, alleviating the technical challenges of implementing coherent anti-Stokes Raman scattering spectroscopy CARS to detect fat stores in living cells. We demonstrate that THG can be used to efficiently and reliably visualize lipid droplets in Caenorhabditis elegans. Thus, THG microscopy offers a versatile alternative to fluorescence and dye-based approaches for lipid biology research.



Author: George J. Tserevelakis , Evgenia V. Megalou , George Filippidis, Barbara Petanidou, Costas Fotakis, Nektarios Tavernarakis

Source: http://plos.srce.hr/



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