A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in ChinaReport as inadecuate




A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in China - Download this document for free, or read online. Document in PDF available to download.

Background

Tuberculosis TB is still a big threat to human health, especially in children. However, an isolation of Mycobacterium tuberculosis culture from pediatric cases remains a challenge. In order to provide some scientific basis for children TB control, we investigated the genotyping and drug resistance characteristics of M. tuberculosis isolates from pediatric cases in China.

Methodology-Principal Findings

In this study, a total of 440 strains including 90 from children <15 years, 159 from adolescents 15–18 years and 191 from adults >18 years isolated in 25 provinces across China were subjected to spoligotyping and drug susceptibility testing. As a result, Beijing family strains were shown to remain predominant in China 85.6%, 81.1% and 75.4% in three above groups, respectively, especially among new children cases 91.0% vs. 69.6% in previously treated cases, P = 0.03. The prevalence of the Beijing genotype isolates was higher in northern and central China in the total collection 85.1% in northern and 83.9% in central vs. 61.6% in southern China, P<0.001 and a similar trend was seen in all three age groups P = 0.708, <0.001 and 0.025, respectively. In adolescents, the frequencies of isoniazid INH-resistant and ethambutol EMB-resistant isolates were significantly higher among Beijing strains compared to non-Beijing genotype strains P = 0.028 for INH and P = 0.027 for EMB. Furthermore, strong association was observed between resistance to rifampicine RIF, streptomycin STR and multidrug resistance MDR among Beijing compared to non-Beijing strains in previously treated cases of children P = 0.01, 0.01 and 0.025, respectively.

Conclusion-Significance

Beijing family was more prevalent in northern and central China compared to southern China and these strains were predominant in all age groups. The genetic diversity of M. tuberculosis isolates from children was similar to that found in adolescents and adults. Beijing genotype was associated with RIF, STR and MDR resistance in previously treated children.



Author: Weiwei Jiao , Zhiguang Liu , Rui Han , Xiuqin Zhao, Fang Dong, Haiyan Dong, Hairong Huang, Jianling Tian, Qinjing Li, Lulu Lian,

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents