Identification of an Additional Minor Pilin Essential for Piliation in the Archaeon Methanococcus maripaludisReportar como inadecuado




Identification of an Additional Minor Pilin Essential for Piliation in the Archaeon Methanococcus maripaludis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Methanococcus maripaludis is an archaeon with two studied surface appendages, archaella and type IV-like pili. Previously, the major structural pilin was identified as MMP1685 and three additional proteins were designated as minor pilins EpdA, EpdB and EpdC. All of the proteins are likely processed by the pilin-specific prepilin peptidase EppA. Six other genes were identified earlier as likely encoding pilin proteins processed also by EppA. In this study, each of the six genes mmp0528, mmp0600, mmp0601, mmp0709, mmp0903 and mmp1283 was deleted and the mutants examined by electron microscopy to determine their essentiality for pili formation. While mRNA transcripts of all genes were detected by RT-PCR, only the deletion of mmp1283 led to nonpiliated cells. This strain could be complemented back to a piliated state by supplying a wildtype copy of the mmp1283 gene in trans. This study adds to the complexity of the type IV pili system in M. maripaludis and raises questions about the functions of the remaining five pilin-like genes and whether M. maripaludis under other growth conditions may be able to assemble additional pili-like structures.



Autor: Divya B. Nair, Daniel K. C. Chung, James Schneider, Kaoru Uchida, Shin-Ichi Aizawa, Ken F. Jarrell

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados