De Novo Lipogenesis and Cholesterol Synthesis in Humans with Long-Standing Type 1 Diabetes Are Comparable to Non-Diabetic IndividualsReportar como inadecuado




De Novo Lipogenesis and Cholesterol Synthesis in Humans with Long-Standing Type 1 Diabetes Are Comparable to Non-Diabetic Individuals - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Synthesis of lipid species, including fatty acids FA and cholesterol, can contribute to pathological disease. The purpose of this study was to investigate FA and cholesterol synthesis in individuals with type 1 diabetes, a group at elevated risk for vascular disease, using stable isotope analysis.

Methods

Individuals with type 1 diabetes n = 9 and age-, sex-, and BMI-matched non-diabetic subjects n = 9 were recruited. On testing day, meals were provided to standardize food intake and elicit typical feeding responses. Blood samples were analyzed at fasting 0 and 24 h and postprandial 2, 4, 6, and 8 hours after breakfast time points. FA was isolated from VLDL to estimate hepatic FA synthesis, whereas free cholesterol FC and cholesteryl ester CE was isolated from plasma and VLDL to estimate whole-body and hepatic cholesterol synthesis, respectively. Lipid synthesis was measured using deuterium incorporation and isotope ratio mass spectrometry.

Results

Fasting total hepatic lipogenesis 3.91±0.90% vs. 5.30±1.22%; P = 0.41 was not significantly different between diabetic and control groups, respectively, nor was synthesis of myristic 28.60±4.90% vs. 26.66±4.57%; P = 0.76, palmitic 12.52±2.75% vs. 13.71±2.64%; P = 0.65, palmitoleic 3.86±0.91% vs. 4.80±1.22%; P = 0.65, stearic 5.55±1.04% vs. 6.96±0.97%; P = 0.29, and oleic acid 1.45±0.28% vs. 2.10±0.51%; P = 0.21. Postprandial lipogenesis was also not different between groups P = 0.38. Similarly, fasting synthesis of whole-body FC 8.2±1.3% vs. 7.3±0.8%-day; P = 0.88 and CE 1.9±0.4% vs. 2.0±0.3%-day; P = 0.96 and hepatic FC 8.2±2.0% vs. 8.1±0.8%-day; P = 0.72 was not significantly different between diabetic and control subjects.

Conclusions

Despite long-standing disease, lipogenesis and cholesterol synthesis was not different in individuals with type 1 diabetes compared to healthy non-diabetic humans.



Autor: Jennifer E. Lambert , Edmond A. Ryan, Alan B. R. Thomson, Michael T. Clandinin

Fuente: http://plos.srce.hr/



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