Altered Lipid Metabolism in Residual White Adipose Tissues of Bscl2 Deficient MiceReport as inadecuate

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Mutations in BSCL2 underlie human congenital generalized lipodystrophy type 2 disease. We previously reported that Bscl2−-− mice develop lipodystrophy of white adipose tissue WAT due to unbridled lipolysis. The residual epididymal WAT EWAT displays a browning phenotype with much smaller lipid droplets LD and higher expression of brown adipose tissue marker proteins. Here we used targeted lipidomics and gene expression profiling to analyze lipid profiles as well as genes involved in lipid metabolism in WAT of wild-type and Bscl2−-− mice. Analysis of total saponified fatty acids revealed that the residual EWAT of Bscl2−-− mice contained a much higher proportion of oleic18:1n9 acid concomitant with a lower proportion of palmitic16:0 acid, as well as increased n3- polyunsaturated fatty acids PUFA remodeling. The acyl chains in major species of triacylglyceride TG and diacylglyceride DG in the residual EWAT of Bscl2−-− mice were also enriched with dietary fatty acids. These changes could be reflected by upregulation of several fatty acid elongases and desaturases. Meanwhile, Bscl2−-− adipocytes from EWAT had increased gene expression in lipid uptake and TG synthesis but not de novo lipogenesis. Both mitochondria and peroxisomal β-oxidation genes were also markedly increased in Bscl2−-− adipocytes, highlighting that these machineries were accelerated to shunt the lipolysis liberated fatty acids through uncoupling to dissipate energy. The residual subcutaneous white adipose tissue ScWAT was not browning but displays similar changes in lipid metabolism. Overall, our data emphasize that, other than being essential for adipocyte differentiation, Bscl2 is also important in fatty acid remodeling and energy homeostasis.

Author: Weiqin Chen , Hongyi Zhou, Siyang Liu, Cassie J. Fhaner, Bethany C. Gross, Todd A. Lydic, Gavin E. Reid



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