Modified Uterine Allotransplantation and Immunosuppression Procedure in the Sheep ModelReportar como inadecuado




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Objective

To develop an orthotopic, allogeneic, uterine transplantation technique and an effective immunosuppressive protocol in the sheep model.

Methods

In this pilot study, 10 sexually mature ewes were subjected to laparotomy and total abdominal hysterectomy with oophorectomy to procure uterus allografts. The cold ischemic time was 60 min. End-to-end vascular anastomosis was performed using continuous, non-interlocking sutures. Complete tissue reperfusion was achieved in all animals within 30 s after the vascular re-anastomosis, without any evidence of arterial or venous thrombosis. The immunosuppressive protocol consisted of tacrolimus, mycophenolate mofetil and methylprednisolone tablets. Graft viability was assessed by transrectal ultrasonography and second-look laparotomy at 2 and 4 weeks, respectively.

Results

Viable uterine tissue and vascular patency were observed on transrectal ultrasonography and second-look laparotomy. Histological analysis of the graft tissue performed in one ewe revealed normal tissue architecture with a very subtle inflammatory reaction but no edema or stasis.

Conclusion

We have developed a modified procedure that allowed us to successfully perform orthotopic, allogeneic, uterine transplantation in sheep, whose uterine and vascular anatomy apart from the bicornuate uterus is similar to the human anatomy, making the ovine model excellent for human uterine transplant research.



Autor: Li Wei , Tao Xue , Hong Yang, Guang-Yue Zhao, Geng Zhang, Zhi-Hong Lu, Yan-Hong Huang, Xiang-Dong Ma, Hai-Xia Liu, Sheng-Ru Liang

Fuente: http://plos.srce.hr/



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