Effects of N-Acetylcysteine in Ozone-Induced Chronic Obstructive Pulmonary Disease ModelReport as inadecuate

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Chronic exposure to high levels of ozone induces emphysema and chronic inflammation in mice. We determined the recovery from ozone-induced injury and whether an antioxidant, N-acetylcysteine NAC, could prevent or reverse the lung damage.


Mice were exposed to ozone 2.5 ppm, 3 hours-12 exposures, over 6 weeks and studied 24 hours 24h or 6 weeks 6W later. Nac 100 mg-kg, intraperitoneally was administered either before each exposure preventive or after completion of exposure therapeutic for 6 weeks.


After ozone exposure, there was an increase in functional residual capacity, total lung volume, and lung compliance, and a reduction in the ratio of forced expiratory volume at 25 and 50 milliseconds to forced vital capacity FEV25-FVC, FEV50-FVC. Mean linear intercept Lm and airway hyperresponsiveness AHR to acetylcholine increased, and remained unchanged at 6W after cessation of exposure. Preventive NAC reduced the number of BAL macrophages and airway smooth muscle ASM mass. Therapeutic NAC reversed AHR, and reduced ASM mass and apoptotic cells.


Emphysema and lung function changes were irreversible up to 6W after cessation of ozone exposure, and were not reversed by NAC. The beneficial effects of therapeutic NAC may be restricted to the ASM.

Author: Feng Li , Cornelis Wiegman , Joanna M. Seiffert, Jie Zhu, Colin Clarke, Yan Chang, Pank Bhavsar, Ian Adcock, Junfeng Zhang, Xin Z

Source: http://plos.srce.hr/


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