Incompatible Nodulation of Bradyrhizobium elkanii Strains BLY3-8 and BLY6-1 with Rj3 Gene-Harboring Soybean CultivarsReportar como inadecuado




Incompatible Nodulation of Bradyrhizobium elkanii Strains BLY3-8 and BLY6-1 with Rj3 Gene-Harboring Soybean Cultivars - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Bradyrhizobiaare known symbiotic partners of soybean. However,some soybean cultivars restrict nodulation by some Bradyrhizobium bacterial strains. These restrictions are related tocompatibility between the Rj genes ofsoybean cultivars and nodulation types of inoculated bacteria. The objective ofthis study was to determine nodulation incompatibility of Type B strains with Rj3 soybean cultivars. Newlyisolated B. elkanii strains BLY3-8 and BLY6-1 from Myanmar and specific strain Bradyrhizobium elkanii USDA33, whichare incompatible with Rj3 soybean cultivars, and B. japonicum USDA110 were used asinoculants to check compatibility or incompatibility with Rj3 soybeancultivars. Nitrogen fixation activity was measured by the acetylene reduction method.Ethylene concentration reduction of acetylene was determined by flame ionizationgas chromatography. According to the inoculation test results, USDA110 was compatible with all soybean cultivars because it formed effectivenodules Figure S1. elkanii strains BLY3-8, BLY6-1, and USDA33 were highly compatible with non-Rj and Rj4-gene harboring soybeancultivars because they had the ability to form functional nodules and possessednitrogenase activity. Inversely, BLY3-8, BLY6-1, and USDA33 were incompatiblewith Rj3 soybean cultivars because they producedineffective nodules. Consequently, the ratio of ineffective nodule number tototal nodule number was >0.5. Therefore, nodule formation by the newlyisolated B. elkanii strains BLY3-8 and BLY6-1 was restricted by the Rj3 soybean cultivars potentially making them useful as specific strains to detectthe Rj3 gene in soybean cultivars.

KEYWORDS

Bradyrhizobium elkanii, Nodulation, Incompatibility, Rj3, Soybean

Cite this paper

Htwe, A. and Yamakawa, T. 2017 Incompatible Nodulation of Bradyrhizobium elkanii Strains BLY3-8 and BLY6-1 with Rj3 Gene-Harboring Soybean Cultivars. American Journal of Plant Sciences, 8, 178-190. doi: 10.4236-ajps.2017.82014.





Autor: Aung Zaw Htwe1,2, Takeo Yamakawa1

Fuente: http://www.scirp.org/



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