Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host InteractionsReportar como inadecuado




Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A Xac, the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen’s adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and-or virulence, such as predicted to encode for xylose repressor-like protein XACΔxylR, Fe-S oxidoredutase XACΔaslB, helicase IV XACΔhelD, ubiquinol cytochrome c oxidoreductase iron-sulfur subunit XACΔpetA, chromosome partitioning protein XACΔparB and cell division protein FtsB XACΔftsB, in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development.

KEYWORDS

Xanthomonas axonopodis Pv. Citri, Mutants, Impaired Virulence

Cite this paper

Ferreira, C. , Moreira, L. , Brigati, J. , Lima, L. , Ferro, J. , Ferro, M. and Oliveira, J. 2017 Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions. Advances in Microbiology, 7, 22-46. doi: 10.4236-aim.2017.71003.





Autor: Cristiano B. Ferreira1,2, Leandro M. Moreira3,4, Joice B. Brigati1, Lonjoré L. Lima5, Jesus A. Ferro1, Maria I. T. Ferro1, Julio

Fuente: http://www.scirp.org/



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