DNase γ Is the Effector Endonuclease for Internucleosomal DNA Fragmentation in NecrosisReportar como inadecuado




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Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation INDF is a biochemical hallmark of apoptosis, and caspase-activated DNase CAD, also known as DNA fragmentation factor 40 kDa DFF40, is one of the major effector endonucleases. DNase γ, a Mg2+-Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that i INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and ii DNase γ, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis.



Autor: Ryushin Mizuta , Shinsuke Araki, Makoto Furukawa, Yuki Furukawa, Syota Ebara, Daisuke Shiokawa, Katsuhiko Hayashi, Sei-ichi Tanum

Fuente: http://plos.srce.hr/



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