The Determination and Evaluation of the Biological Activities for the Commercialization of Recombinant Follicle-Stimulating Hormone in VitroReportar como inadecuado




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Follicle-stimulating hormone FSH plays a central role in mammals reproduction, with the actions of FSH mediated by follicle-stimulating hormone receptors FSHRs on the surface of target cells. The purposes of this study were to determine and evaluate the biological activities for the commercialization of recombinant follicle-stimulating hormone rFSH in vitro through the cellular internalization using cloned 293T-FSHR cell lines as target. Using imaging approaches we have found here that a little fluorescent signal from the surface of the cell transferred to the cytoplasm and accumulated around the nucleus by endocytosis. Compared with the control groups, the commercialization of rFSH have not the significant differences of internalization, but the rFSH have promoted the internalization of the fluorescent, suggested that this detection system might as a protocol for the bioactivity of recombinant therapeutic proteins in vitro.

KEYWORDS

Recombinant Follicle-Stimulating Hormone rFSH, Follicle-Stimulating Hormone ReceptorFSHR , Cellular Internalization

Cite this paper

Hu, J. , Han, J. , Zhang, X. , Chen, F. , Liu, L. and Zeng, B. 2016 The Determination and Evaluation of the Biological Activities for the Commercialization of Recombinant Follicle-Stimulating Hormone in Vitro. Journal of Biosciences and Medicines, 4, 31-36. doi: 10.4236-jbm.2016.412005.





Autor: Jianwen Hu, Jizhong Han, Xian Zhang, Fei Chen, Lanlan Liu, Bin Zeng*

Fuente: http://www.scirp.org/



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