Simvastatin Action Is Not Related to HDAC2 Expression in Non-Small Cell Lung Cancer NSCLCReportar como inadecuado




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In this study, we lowered the expression of HDAC2protein, to evaluate the effects of simvastatin on the biochemical pathwaysinvolved in inflammatory and metastatic response. The model used is thenon-small cell lung cancer line GLC-82. Trypanblue staining for assessing vital cell number to be seed andMTT assay was used as cell proliferation test. Lentivus for HDAC2 was used to silence its mRNA. Western blotting analysis was used for protein extracts, andELISA was done that Lentivirus containing theshHDAC2 in GLC-82 cells was able to reduce protein expression of HDAC2.In the GLCshHDAC2 cell line obtained, H2O2 induced asignificant increase in cytokines release and ERK1-2 phosphorylation P 0.01;a significant decrease of RECK activation P 0.01;a significant increased activation P In conclusion, from ourreport emerge, that simvastatin is able perse to inhibit oxidative stress in lung cancer cells, overcoming HDAC2expression.

KEYWORDS

Lung Adenocarcinoma Cells, HDAC2, Statins, Cell Proliferation, Cytokines

Cite this paper

Luca, G. , Daniela, F. , Mariarita, P. , Cione, E. , Girolamo, P. , Maria, M. , Rosa, T. , Giuseppe, S. , Bruno, D. , Michele, N. and Rocco, S. 2016 Simvastatin Action Is Not Related to HDAC2 Expression in Non-Small Cell Lung Cancer NSCLC. Journal of Cancer Therapy, 7, 939-952. doi: 10.4236-jct.2016.712091.





Autor: Gallelli Luca1, Falcone Daniela1, Perri Mariarita2, Erika Cione2, Pelaia Girolamo1, Mesuraca Maria3, Terracciano Rosa1, Spaziano

Fuente: http://www.scirp.org/



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