Rhizoctonia Bataticola Lectin RBL Induces Caspase-8-Mediated Apoptosis in Human T-Cell Leukemia Cell Lines but Not in Normal CD3 and CD34 Positive CellsReportar como inadecuado




Rhizoctonia Bataticola Lectin RBL Induces Caspase-8-Mediated Apoptosis in Human T-Cell Leukemia Cell Lines but Not in Normal CD3 and CD34 Positive Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

We have previously demonstrated immunostimulatory activity of a fungal lectin, Rhizoctonia bataticola lectin RBL, towards normal human peripheral blood mononuclear cells. The present study aimed to explore the anticancer activities of RBL using human leukemic T-cell lines, Molt-4, Jurkat and HuT-78. RBL exhibited significant binding >90% to the cell membrane that was effectively inhibited by complex glycoproteins such as mucin 97% inhibition and asialofetuin 94% inhibition but not simple sugars such as N-acetyl-D-galactosamine, glucose and sucrose. RBL induced a dose and time dependent inhibition of proliferation and induced cytotoxicity in the cell lines. The percentage of apoptotic cells, as determined by hypodiploidy, was 33% and 42% in Molt-4 and Jurkat cells, respectively, compared to 3.11% and 2.92% in controls. This effect was associated with a concomitant decrease in the G0-G1 population. Though initiator caspase-8 and -9 were activated upon exposure to RBL, inhibition of caspase-8 but not caspase-9 rescued cells from RBL-induced apoptosis. Mechanistic studies revealed that RBL induced cleavage of Bid, loss of mitochondrial membrane potential and activation of caspase-3. The expression of the anti-apoptotic proteins Bcl-2 and Bcl-X was down regulated without altering the expression of pro-apoptotic proteins- Bad and Bax. In contrast to leukemic cells, RBL did not induce apoptosis in normal PBMC, isolated CD3+ve cells and undifferentiated CD34+ve hematopoietic stem and progenitor cells HSPCs. The findings highlight the differential effects of RBL on transformed and normal hematopoietic cells and suggest that RBL may be explored for therapeutic applications in leukemia.



Autor: Radha Pujari, Sachin M. Eligar, Natesh Kumar, Srikanth Barkeer, Vishwanath Reddy, Bale M. Swamy, Shashikala R. Inamdar, Padma Sha

Fuente: http://plos.srce.hr/



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