Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based MetabolomicsReportar como inadecuado




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Background

Primary focal segmental glomerulosclerosis FSGS is pathological entity which is characterized by idiopathic steroid-resistant nephrotic syndrome SRNS and progression to end-stage renal disease ESRD in the majority of affected individuals. Currently, there is no practical noninvasive technique to predict different pathological types of glomerulopathies. In this study, the role of urinary metabolomics in the diagnosis and pathogenesis of FSGS was investigated.

Methods

NMR-based metabolomics was applied for the urinary metabolic profile in the patients with FSGS n = 25, membranous nephropathy MN, n = 24, minimal change disease MCD, n = 14 and IgA nephropathy IgAN, n = 26, and healthy controls CON, n = 35. The acquired data were analyzed using principal component analysis PCA followed by orthogonal projections to latent structure discriminant analysis OPLS-DA. Model validity was verified using permutation tests.

Results

FSGS patients were clearly distinguished from healthy controls and other three types of glomerulopathies with good sensitivity and specificity based on their global urinary metabolic profiles. In FSGS patients, urinary levels of glucose, dimethylamine and trimethylamine increased compared with healthy controls, while pyruvate, valine, hippurate, isoleucine, phenylacetylglycine, citrate, tyrosine, 3-methylhistidine and β-hydroxyisovalerate decreased. Additionally, FSGS patients had lower urine N-methylnicotinamide levels compared with other glomerulopathies.

Conclusions

NMR-based metabonomic approach is amenable for the noninvasive diagnosis and differential diagnosis of FSGS as well as other glomerulopathies, and it could indicate the possible mechanisms of primary FSGS.



Autor: Xu Hao , Xia Liu , Weiming Wang, Hong Ren, Jingyuan Xie, Pingyan Shen, Donghai Lin, Nan Chen

Fuente: http://plos.srce.hr/



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