Research of Anti-Cancer Components in Traditional Chinese Medicine on Hollow Fibre Cell Fishing and Hollow Fibre Liquid Phase MicroextractionReportar como inadecuado




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Hollowfibre cell fishing with HPLC HFCF-HPLC based on the human ovarian cancer cellline SKOV-3, human renal tubular cell line ACHN or hepatoma cell line HepG-2was employed to screen active groups of coumarin and volatile oil in Radix angelicae sinensis, Radix angelicae dahuricae and Fructus citri sarcodactylis. Simultaneously,hollow fibre liquid phase microextraction with HPLC HFLPME-HPLC was conductedto enrich and determine the contents of active components in the same samplesolution. Before application, for HFCF-HPLC, cells growth states and survivalrates on the fibre, effect of ethanol concentration in the extract of sampleson cell survival rates, non-specific binding between fibre active centres andthe target components, positive and negative controls and repeatabilities werevalidated; for HFLPME, extraction solvent, sample phase pH, agitation speed,extraction time and sample phase volume were investigated. Many active components were screened from three medicines. Someof them, such as scoparone, psoralen, bergapten, oxypeucedanin, imperatorin,ligustilide, were identified by MS. The target fishing factors of active componentsand the cell apoptosis rates of three cells under the medicines effect were researched.The binding sites of active groups on HepG-2 cells were preliminarily determined.The results demonstrated that HFCF-HPLC, coupled with HFLPME-HPLC, is a simpleand universal approach to find bioactive components at the cellular level,determine their content and research traditional Chinese medicines TCMs entiretyeffect of multi-component and multi-target. The approach may provide us a newand good solution to clarify the material basis of anti-cancer effect andconduct personalized quality control for the components associated withefficacy in TCMs.

KEYWORDS

Hollow Fibre Cell Fishing, Liquid Phase Microextraction, Identification, Quantification, Cancer Cell, Activity Screening

Cite this paper

Li, F. , Chen, X. , Xu, Y. and Bai, X. 2016 Research of Anti-Cancer Components in Traditional Chinese Medicine on Hollow Fibre Cell Fishing and Hollow Fibre Liquid Phase Microextraction. American Journal of Analytical Chemistry, 7, 696-711. doi: 10.4236-ajac.2016.710063.





Autor: Feixue Li, Xuan Chen, Yanli Xu, Xiaohong Bai*

Fuente: http://www.scirp.org/



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