A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis CReportar como inadecuado




A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Approximately 170 million people are chronic carriers of hepatitis C virus HCV. Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin PEG-IFN-RBV. A genome-wide association with PEG-IFN-RBV treatment response and a single nucleotide polymorphism rs12979860 has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods.



Autor: Camila da Silva Ferreira, Rodrigo Martins Abreu, Marlone Cunha da Silva, Aline Siqueira Ferreira, Paulo Dominguez Nasser, Flair J

Fuente: http://plos.srce.hr/



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