Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thalianaReportar como inadecuado




Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thaliana - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Mammalian sex hormones are spread in the environmentfrom natural and anthropogenic sources. In the present study, the effect ofestradiol on Arabidopsis thaliana growth primary metabolism, phenylpropanoid and flavonoid pathways and pathogenresistance were investigated. Treatments of Arabidopsis plants with 10 and 100 nM 17β-estradiolresulted in enhanced root growth and shoot biomass. In addition, treated plantshad an increased rate of photosynthesis with a concomitant increase in carbohydrateand protein accumulation. Plants exposed to higher concentrations of 17β-estradiol 10 μM had significantlylower root growth, biomass, photosynthesis rate, primary metabolite andphenylpropanoid and flavonoid contents indicating a toxic effect of estradiol.Treatments with increasing estradiol concentrations 10 nM, 100 nM and 10 μMresulted in the downregulation of phenylpropanoid-flavonoid pathway genes PAL1, PAL4, CHI and ANS and subsequent decreasedaccumulation of phenolics, flavonoids and anthocyanins. Estradiol-treatedplants were inoculated with Pseudomonassyringae pv. Tomato DC3000 and basal resistance was determined. Estradioltreatments rendered plants susceptible to the pathogen, thus compromising theplant defense mechanisms. These results indicate that at low concentrations,estradiol functions as a biostimulant of growth, yield and primary metabolismof Arabidopsis. However, estradiolfunctions as a potential transcriptional regulator of the phenylpropanoidpathway genes in Arabidopsis, havinga negative effect on the phenylpropanoid and flavonoid biosynthetic pathways.

KEYWORDS

Plant Ecology, Plant Physiology, Plant Host-Pathogen Interactions

Cite this paper

Upadhyay, P. and Maier, C. 2016 Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thaliana. American Journal of Plant Sciences, 7, 1693-1710. doi: 10.4236-ajps.2016.713160.





Autor: Pallavi Upadhyay, Camelia Maier

Fuente: http://www.scirp.org/



DESCARGAR PDF




Documentos relacionados