Transcriptional Reprogramming of CD11b Esamhi Dendritic Cell Identity and Function by Loss of Runx3Reportar como inadecuado




Transcriptional Reprogramming of CD11b Esamhi Dendritic Cell Identity and Function by Loss of Runx3 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Classical dendritic cells cDC are specialized antigen-presenting cells mediating immunity and tolerance. cDC cell-lineage decisions are largely controlled by transcriptional factor regulatory cascades. Using an in vivo cell-specific targeting of Runx3 at various stages of DC lineage development we show that Runx3 is required for cell-identity, homeostasis and function of splenic Esamhi DC. Ablation of Runx3 in DC progenitors led to a substantial decrease in splenic CD4+-CD11b+ DC. Combined chromatin immunoprecipitation sequencing and gene expression analysis of purified DC-subsets revealed that Runx3 is a key gene expression regulator that facilitates specification and homeostasis of CD11b+Esamhi DC. Mechanistically, loss of Runx3 alters Esamhi DC gene expression to a signature characteristic of WT Esamlow DC. This transcriptional reprogramming caused a cellular change that diminished phagocytosis and hampered Runx3- Esamhi DC capacity to prime CD4+ T cells, attesting to the significant role of Runx3 in specifying Esamhi DC identity and function.



Autor: Joseph Dicken, Alexander Mildner, Dena Leshkowitz, Ivo P. Touw, Shay Hantisteanu, Steffen Jung, Yoram Groner

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados