Intracellular Regulation of Cross-Presentation during Dendritic Cell MaturationReportar como inadecuado

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We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells DCs to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-α, CpG and LPS, but was less affected upon CD40L-induced maturation. The difference could not be explained by decreased antigen uptake or translocation into the cytosol, but decreased cross-presentation ability did correlate with increased phagosomal-lysosomal acidification. Nevertheless, intra-phagosomal degradation of OVA was not increased in matured samples, suggesting that decreasing phagosomal pH may also regulate cross-presentation by a mechanism other than enhancing degradation.

Autor: Claudia S. Wagner, Jeff Grotzke, Peter Cresswell



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