In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatumReportar como inadecuado




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Plant growth hormoneBAP benzyl amino purine, KIN kinetin, NAA 1-naphthalene acetic acid andIBA indole-3 butyric acid effect was studied on in vitro multiplication of shoots and rooting of Drepanostachyum falcatum. In vitro micropropagation of himalayanweeping bamboo is explained by in vitro shoot induction and proliferation. Excised explant with axillary bud is surfacesterilized with 0.1% HgCl2 for 10 - 12 minutes, cleaned with 90%ethanol and inoculated on liquid Murashige and Skoog MS culture mediumsupplemented with different concentrations of BAP- KIN. Effect of BAP-KIN onshoot induction is with different rate and number of shoots produced byexplants with axillary bud cultured on MS media supplemented with 0.0 mg-LBAP-KIN - 5.5 mg-L BAP-KIN. Shoot multiplication with highest rate is achievedon MS medium supplemented with 3.5 mg-L BAP after 4th sub-culturing.The most effective with highest rate and number of root induction combination is6.5 mg-L IBA after 5 weeks. The roots produced by 6.5 mg-L IBA is best comparedwith other combination of auxin NAA 1-naphthalene acetic acid.

KEYWORDS

In Vitro, Drepanostachyum falcatum, Himalayan Weeping Bamboo, Plant Growth Hormone, Micropropagation

Cite this paper

Saini, H. , Arya, I. , Arya, S. and Sharma, R. 2016 In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum. American Journal of Plant Sciences, 7, 1317-1324. doi: 10.4236-ajps.2016.79126.





Autor: Himanshu Saini1,2, Inder Dev Arya1, Sarita Arya1, Reetu Sharma1

Fuente: http://www.scirp.org/



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