Determination of the Fatty Acid Profile of Breast Milk from Nursing Mothers in Bungoma County, KenyaReportar como inadecuado




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The fatty acid profile in breast milk of nursing mothers who participated in a Cohort for Vitamin A COVA study at the fourth and ninth month of lactation was investigated. Breast milk samples were collected by manual expression and stored at - 20°C until analysis. The fat was extracted from the milk and methylated using the American Oil Chemists’ Society AOCS Official Methods with modifications. The separation, identification and quantification of the fatty acid methyl esters was performed by gas chromatography coupled with mass spectrometry GC-MS. Fat contents of human milk increased significantly between the fourth and ninth month of lactation 0.38 and 1.21 mg·mL-1 respectively; P < 0.05. The major fatty acids of breast milk fat at the 4th month of lactation were oleic acid 66.6%, followed by linoleic acid 51.3% and palmitoleic acid 28.9%. However, at the 9th month, the major component was linoleic acid 58.1%, followed by oleic acid 30.9% and stearic acid 23.1%. The percentage average of the total saturated fatty acids at the 4th month 16.62% was significantly lower P



Autor: Virate J. Kiprop1, Amy W. Girard2, Lilian A. Gogo1, Mary N. Omwamba1, Symon M. Mahungu1*

Fuente: http://www.scirp.org/



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