Mutation of the Light-Induced Yellow Leaf 1 Gene, Which Encodes a Geranylgeranyl Reductase, Affects Chlorophyll Biosynthesis and Light Sensitivity in RiceReportar como inadecuado




Mutation of the Light-Induced Yellow Leaf 1 Gene, Which Encodes a Geranylgeranyl Reductase, Affects Chlorophyll Biosynthesis and Light Sensitivity in Rice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Chlorophylls Chls are crucial for capturing light energy for photosynthesis. Although several genes responsible for Chl biosynthesis were characterized in rice Oryza sativa, the genetic properties of the hydrogenating enzyme involved in the final step of Chl synthesis remain unknown. In this study, we characterized a rice light-induced yellow leaf 1-1 lyl1-1 mutant that is hypersensitive to high-light and defective in the Chl synthesis. Light-shading experiment suggested that the yellowing of lyl1-1 is light-induced. Map-based cloning of LYL1 revealed that it encodes a geranylgeranyl reductase. The mutation of LYL1 led to the majority of Chl molecules are conjugated with an unsaturated geranylgeraniol side chain. LYL1 is the firstly defined gene involved in the reduction step from Chl-geranylgeranylated ChlGG and geranylgeranyl pyrophosphate GGPP to Chl-phytol ChlPhy and phytyl pyrophosphate PPP in rice. LYL1 can be induced by light and suppressed by darkness which is consistent with its potential biological functions. Additionally, the lyl1-1 mutant suffered from severe photooxidative damage and displayed a drastic reduction in the levels of α-tocopherol and photosynthetic proteins. We concluded that LYL1 also plays an important role in response to high-light in rice.



Autor: Yong Zhou , Zhiyun Gong , Zefeng Yang , Yuan Yuan, Jinyan Zhu, Man Wang, Fuhai Yuan, Shujun Wu, Zhiqin Wang, Chuandeng Yi, Tinghu

Fuente: http://plos.srce.hr/



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