CIP2A Influences Survival in Colon Cancer and Is Critical for Maintaining Myc ExpressionReportar como inadecuado

CIP2A Influences Survival in Colon Cancer and Is Critical for Maintaining Myc Expression - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The cancerous inhibitor of protein phosphatase 2A CIP2A is an oncogenic factor that stabilises the c-Myc protein. CIP2A is overexpressed in several tumours, and expression levels are an independent marker for long-term outcome. To determine whether CIP2A expression is elevated in colon cancer and whether it might serve as a prognostic marker for survival, we analysed CIP2A mRNA expression by real-time PCR in 104 colon cancer samples. CIP2A mRNA was overexpressed in colon cancer samples and CIP2A expression levels correlated significantly with tumour stage. We found that CIP2A serves as an independent prognostic marker for disease-free and overall survival. Further, we investigated CIP2A-dependent effects on levels of c-Myc, Akt and on cell proliferation in three colon cancer cell lines by silencing CIP2A using small interfering si and short hairpin sh RNAs. Depletion of CIP2A substantially inhibited growth of colon cell lines and reduced c-Myc levels without affecting expression or function of the upstream regulatory kinase, Akt. Expression of CIP2A was found to be dependent on MAPK activity, linking elevated c-Myc expression to deregulated signal transduction in colon cancer.

Autor: Armin Wiegering , Christina Pfann, Friedrich Wilhelm Uthe, Christoph Otto, Lukas Rycak, Uwe Mäder, Martin Gasser, Anna-Maria Waa



Documentos relacionados