Detection and Transfer of Extended Spectrum Beta Lactamase Enzymes from Untreated Hospital Waste WaterReportar como inadecuado




Detection and Transfer of Extended Spectrum Beta Lactamase Enzymes from Untreated Hospital Waste Water - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Untreated Hospitalwastewater piped into septic tanks contributes to the spread of antibiotic resistancein developing countries. This study was conducted to determine the resistantprofile, and Extended Spectrum Beta-Lactamases ESBLs production in isolatesfrom hospital waste water, of 2 hospitals in Delta State, Nigeria. A total of147 organisms were isolated from 32 waste water samples. One hundred and twentythree isolates were Gram negative and 24 were Gram positive. Escherichia coli was the most prevalentin the two locations. Antimicrobial susceptibility by standard disk diffusionmethod was performed. All isolates were resistant to 4 or more antimicrobialagents. Out of the 123 Gram negative Bacteria, 33 were pan drug resistant andwere selected for plasmid curing, DNA extraction and phenotypic detection ofESBL. Transfer of resistant by broth mating technique was performed. Plasmidcuring and extraction result indicated that isolates carried resistance on theplasmid and harboured similar multiple high molecular weight plasmids of 23.13 kband 9.4 kb. ESBL production was detected in 15.15%. Transfer of resistant genesbetween ESBL producing and non-ESBL producing isolates was observed. Incidenceof ESBL in untreated hospital waste water haspublic health implications. Therefore establishment of treatment plants inour hospital is paramount in achieving sustainable health.

KEYWORDS

Waste Water, Plasmids, ESBL, Escherichia coli

Cite this paper

Egbule, O. 2016 Detection and Transfer of Extended Spectrum Beta Lactamase Enzymes from Untreated Hospital Waste Water. Advances in Microbiology, 6, 512-520. doi: 10.4236-aim.2016.67051.





Autor: Olivia Sochi Egbule

Fuente: http://www.scirp.org/



DESCARGAR PDF




Documentos relacionados