Platelet-Derived Stromal Cell-Derived Factor-1 Is Required for the Transformation of Circulating Monocytes into Multipotential CellsReportar como inadecuado




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Background

We previously described a primitive cell population derived from human circulating CD14+ monocytes, named monocyte-derived multipotential cells MOMCs, which are capable of differentiating into mesenchymal and endothelial lineages. To generate MOMCs in vitro, monocytes are required to bind to fibronectin and be exposed to soluble factors derived from circulating CD14− cells. The present study was conducted to identify factors that induce MOMC differentiation.

Methods

We cultured CD14+ monocytes on fibronectin in the presence or absence of platelets, CD14− peripheral blood mononuclear cells, platelet-conditioned medium, or candidate MOMC differentiation factors. The transformation of monocytes into MOMCs was assessed by the presence of spindle-shaped adherent cells, CD34 expression, and the potential to differentiate in vitro into mesenchymal and endothelial lineages.

Results

The presence of platelets or platelet-conditioned medium was required to generate MOMCs from monocytes. A screening of candidate platelet-derived soluble factors identified stromal cell-derived factor SDF-1 as a requirement for generating MOMCs. Blocking an interaction between SDF-1 and its receptor CXCR4 inhibited MOMC generation, further confirming SDF-1′s critical role in this process. Finally, circulating MOMC precursors were found to reside in the CD14+CXCR4high cell population.

Conclusion

The interaction of SDF-1 with CXCR4 is essential for the transformation of circulating monocytes into MOMCs.



Autor: Noriyuki Seta, Yuka Okazaki, Hiroshi Miyazaki, Takashi Kato, Masataka Kuwana

Fuente: http://plos.srce.hr/



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