Expression Analysis of Aldo-Keto Reductase 1 AKR1 in Foxtail Millet Setaria italica L. Subjected to Abiotic StressesReportar como inadecuado




Expression Analysis of Aldo-Keto Reductase 1 AKR1 in Foxtail Millet Setaria italica L. Subjected to Abiotic Stresses - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Foxtail millet Setaria italica L. is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases AKRs are significant part of plant defence mechanism, having an ability to confer multiple stress tolerance. In this study, AKR1 gene expression was studied in roots and leaves of foxtail millet subjected to different regimes of PEG- and NaCl-stress for seven days. The quantitative Real-time PCR expression analysis in both root and leaves showed upregulation of AKR1 gene during PEG and salt stress. A close correlation exits between expression of AKR1 gene and the rate of lipid peroxidation along with the retardation of growth. Tissue-specific differences were found in the AKR1 gene expression to the stress intensities studied. The reduction in root and shoot growth under both stress conditions were dependent on stress severity. The level of lipid peroxidation as indicated by MDA formation was significantly increased in roots and leaves along with increased stress levels. Finally, these findings support the early responsive nature of AKR1 gene and seem to be associated at least in part with its ability to contribute in antioxidant defence related pathways which could provide a better protection against oxidative stress under stress conditions.

KEYWORDS

Aldo-Keto Reductase, Lipid Peroxidation, Reactive Carbonyls, Cellular Homeostasis, Plant Abiotic Stress Response

Cite this paper

Kirankumar, T. , Madhusudhan, K. , Nareshkumar, A. , Kiranmai, K. , Lokesh, U. , Venkatesh, B. and Sudhakar, C. 2016 Expression Analysis of Aldo-Keto Reductase 1 AKR1 in Foxtail Millet Setaria italica L. Subjected to Abiotic Stresses. American Journal of Plant Sciences, 7, 500-509. doi: 10.4236-ajps.2016.73044.





Autor: Tanguturi Venkata Kirankumar, Kalaiahgari Venkata Madhusudhan, Ambekar Nareshkumar, Kurnool Kiranmai, Uppala Lokesh, Boya Venkate

Fuente: http://www.scirp.org/



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