Comparative Evaluation of GenoType MTBDRplus Line Probe Assay with Solid Culture Method in Early Diagnosis of Multidrug Resistant Tuberculosis MDR-TB at a Tertiary Care Centre in IndiaReportar como inadecuado




Comparative Evaluation of GenoType MTBDRplus Line Probe Assay with Solid Culture Method in Early Diagnosis of Multidrug Resistant Tuberculosis MDR-TB at a Tertiary Care Centre in India - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

The objectives of the study were to compare the performance of line probe assay GenoType MTBDRplus with solid culture method for an early diagnosis of multidrug resistant tuberculosis MDR-TB, and to study the mutation patterns associated with rpoB, katG and inhA genes at a tertiary care centre in north India.

Methods

In this cross-sectional study, 269 previously treated sputum-smear acid-fast bacilli AFB positive MDR-TB suspects were enrolled from January to September 2012 at the All India Institute of Medical Sciences hospital, New Delhi. Line probe assay LPA was performed directly on the sputum specimens and the results were compared with that of conventional drug susceptibility testing DST on solid media Lowenstein Jensen LJ method.

Results

DST results by LPA and LJ methods were compared in 242 MDR-TB suspects. The LPA detected rifampicin RIF resistance in 70 of 71 cases, isoniazid INH resistance in 86 of 93 cases, and MDR-TB in 66 of 68 cases as compared to the conventional method. Overall rifampicin, isoniazid and MDR-TB concordance of the LPA with the conventional DST was 96%. Sensitivity and specificity were 98% and 99% respectively for detection of RIF resistance; 92% and 99% respectively for detection of INH resistance; 97% and 100% respectively for detection of MDR-TB. Frequencies of katG gene, inhA gene and combined katG and inhA gene mutations conferring all INH resistance were 72-87 83%, 10-87 11% and 5-87 6% respectively. The turnaround time of the LPA test was 48 hours.

Conclusion

The LPA test provides an early diagnosis of monoresistance to isoniazid and rifampicin and is highly sensitive and specific for an early diagnosis of MDR-TB. Based on these findings, it is concluded that the LPA test can be useful in early diagnosis of drug resistant TB in high TB burden countries.



Autor: Raj N. Yadav, Binit K. Singh, Surendra K. Sharma , Rohini Sharma, Manish Soneja, Vishnubhatla Sreenivas, Vithal P. Myneedu, Mahmu

Fuente: http://plos.srce.hr/



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