Molecular Characterization of Poxviruses Associated with Tattoo Skin Lesions in UK CetaceansReportar como inadecuado




Molecular Characterization of Poxviruses Associated with Tattoo Skin Lesions in UK Cetaceans - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

There is increasing concern for the well-being of cetacean populations around the UK. Tattoo skin disease characterised by irregular, grey, black or yellowish, stippled cutaneous lesions caused by poxvirus infection is a potential health indicatora potential health indicator for cetaceans. Limited sequence data indicates that cetacean poxviruses CPVs belong to an unassigned genus of the Chordopoxvirinae. To obtain further insight into the phylogenetic relationships between CPV and other Chordopoxvirinae members we partially characterized viral DNA originating from tattoo lesions collected in Delphinidae and Phocoenidae stranded along the UK coastline in 1998–2008. We also evaluated the presence of CPV in skin lesions other than tattoos to examine specificity and sensitivity of visual diagnosis. After DNA extraction, regions of the DNA polymerase and DNA topoisomerase I genes were amplified by PCR, sequenced and compared with other isolates. The presence of CPV DNA was demonstrated in tattoos from one striped dolphin Stenella coeruleoalba, eight harbour porpoises Phocoena phocoena and one short-beaked common dolphin Delphinus delphis and in one ‘dubious tattoo’ lesion detected in one other porpoise. Seventeen of the 18 PCR positive skin lesions had been visually identified as tattoos and one as a dubious tattoo. None of the other skin lesions were PCR positive. Thus, visual identification had a 94.4% sensitivity and 100% specificity. The DNA polymerase PCR was most effective in detecting CPV DNA. Limited sequence phylogeny grouped the UK samples within the odontocete poxviruses CPV group 1 and indicated that two different poxvirus lineages infect the Phocoenidae and the Delphinidae. The phylogenetic tree had three major branches: one with the UK Phocoenidae viruses, one with the Delphinidae isolates and one for the mysticete poxvirus CPV group 2. This implies a radiation of poxviruses according to the host suborder and the families within these suborders.



Autor: Barbara A. Blacklaws , Anna M. Gajda, Sabine Tippelt, Paul D. Jepson, Rob Deaville, Marie-Francoise Van Bressem, Gareth P. Pearce

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados