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Bingqian Shen ;Electronic Journal of Biotechnology 2010, 13 5

Autor: Qiangyi Fang

Fuente: http://www.redalyc.org/


Introducción



Electronic Journal of Biotechnology E-ISSN: 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile Fang, Qiangyi; Shen, Bingqian Optimization of polyethylenimine-mediated transient transfection using response surface methodology design Electronic Journal of Biotechnology, vol.
13, núm.
5, 2010, pp.
1-11 Pontificia Universidad Católica de Valparaíso Valparaíso, Chile Available in: http:--www.redalyc.org-articulo.oa?id=173318799010 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Electronic Journal of Biotechnology ISSN: 0717-3458 http:--www.ejbiotechnology.info DOI: 10.2225-vol13-issue5-fulltext-6 Optimization of polyethylenimine-mediated transient transfection using response surface methodology design Qiangyi Fang1 · Bingqian Shen2 1 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China 2 College of Pharmaceutical Sciences, Nankai University, Tianjin, China Corresponding author: hicell@gmail.com Received March 6, 2010 - Accepted May 26, 2010 Published online: September 15, 2010 © 2010 by Pontificia Universidad Católica de Valparaíso, Chile Abstract Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells.
A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration and incubation time on transient transfection efficiency.
The highest integral optic density of green fluorescent protein presenting r-protein yield was accessed using a DNA concentration of 1.75 µg-mL, a polyethylenimine concentration of 10.5 µg-mL, and an incubation time of 11.8 min.
Analysis of variance demonstrated...





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