Optimization in agrobacterium-mediated transformation of anthurium andraeanum using gfp as a reporter Report as inadecuate




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Ji Jing ; Gang Wang ; Jie Hua Wang ; Yuan Yuan Feng ; Han Wen Xing ; Chun Feng Guan ;Electronic Journal of Biotechnology 2010, 13 5

Author: Qing Zhao

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Electronic Journal of Biotechnology E-ISSN: 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile Zhao, Qing; Jing, Ji; Wang, Gang; Hua Wang, Jie; Yuan Feng, Yuan; Wen Xing, Han; Feng Guan, Chun Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter Electronic Journal of Biotechnology, vol.
13, núm.
5, 2010, pp.
1-12 Pontificia Universidad Católica de Valparaíso Valparaíso, Chile Available in: http:--www.redalyc.org-articulo.oa?id=173318799009 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Electronic Journal of Biotechnology ISSN: 0717-3458 http:--www.ejbiotechnology.info DOI: 10.2225-vol13-issue5-fulltext-2 Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter 1 2 Qing Zhao · Ji Jing 2 2 · Gang Wang 2 2 · Jie Hua Wang · Yuan Yuan Feng 2 Han Wen Xing · Chun Feng Guan 1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China 2 School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, PR China Corresponding author: jijing@tju.edu.cn - gangwang@tju.edu.cn Received September 16, 2009 - Accepted May 5, 2010 Published online: September 15, 2010 © 2010 by Pontificia Universidad Católica de Valparaíso, Chile Abstract Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging.
In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene.
The possibility of fa...





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