Spectrophotometric Study for the Reaction of Pentoxifylline Hydrochloride with 1,2-Naphthoquinone-4-Sulphonate: Kinetics, Mechanism and Application for Development of High-Throughput Kinetic Microwell Assay for Pentoxifylline in Reportar como inadecuado




Spectrophotometric Study for the Reaction of Pentoxifylline Hydrochloride with 1,2-Naphthoquinone-4-Sulphonate: Kinetics, Mechanism and Application for Development of High-Throughput Kinetic Microwell Assay for Pentoxifylline in - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Spectrophotometricstudy was carried out, for the first time, to investigate the reaction betweenthe vasodilator pentoxifylline hydrochloride POX and1,2-naphthoquinone-4-sulphonate NQS reagent. The reaction occurs in alkalinemedium to activate the nucleophilic substitution reaction producing anorange-colored product measured spectrophometrically at λmax 472 nm. The variables affecting the reaction werecarefully studied and the conditions were optimized. The kinetics of thereaction was investigated and its activation energy was found to be 0.262cal-mol. Owing to its low activation energy, the reaction proceeded easily andwas successfully used for simple and rapid assay of POX. The stoichiometry ofthe reaction was determined 1:1, and the reaction mechanism was suggested. Todevelop a high-throughput methodology used in quality control laboratory, acomparative study of the reaction using the conventional spectrophotometricversus microwell assay was applied. Under the optimum reaction conditions, theinitial rate and fixed time methods were utilized for constructing the calibrationgraphs for determination of POX concentrations. The linear range was 10 - 120 μg-ml with good correlation coefficients 0.9987 -0.9998. The LOD was 2.5 and 3.4 μg-ml for initial rate and fixed time methods, respectively. The intra-and inter-day accuracy and precision of the developed methods were satisfactory,where RSD was ≤3.94%. The present methods have been successfully applied to thedetermination of POX in its pharmaceutical tablets, and the percentage recoveryvalues were 97.9% - 101.9%. Therefore, we strongly recommend the proposedmethods for determination of POX in quality control laboratories.

KEYWORDS

Pentoxifylline HCl, 1, 2-Naphthoquinone-4-Sulphonate, Kinetic Spectrophotometry, Pharmaceutical Analysis, Micro-Well Plate Reader

Cite this paper

Mahmoud, A. and AlQahtani, S. 2016 Spectrophotometric Study for the Reaction of Pentoxifylline Hydrochloride with 1,2-Naphthoquinone-4-Sulphonate: Kinetics, Mechanism and Application for Development of High-Throughput Kinetic Microwell Assay for Pentoxifylline in Quality Control Laboratory. American Journal of Analytical Chemistry, 7, 179-191. doi: 10.4236-ajac.2016.72015.





Autor: Ashraf M. Mahmoud1,2*, Saad A. AlQahtani3

Fuente: http://www.scirp.org/



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