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Zhiqing Huang ; Bo Zhou ; Yanliu Luo ; Gang Jia ; Guangmang Liu ; Hua Zhao ; Xiaoling Chen ;Electronic Journal of Biotechnology 2015, 18 5

Autor: Min Zhou

Fuente: http://www.redalyc.org/


Introducción



Electronic Journal of Biotechnology E-ISSN: 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile Zhou, Min; Huang, Zhiqing; Zhou, Bo; Luo, Yanliu; Jia, Gang; Liu, Guangmang; Zhao, Hua; Chen, Xiaoling Construction and expression of two-copy engineered yeast of feruloyl esterase Electronic Journal of Biotechnology, vol.
18, núm.
5, 2015, pp.
338-342 Pontificia Universidad Católica de Valparaíso Valparaíso, Chile Available in: http:--www.redalyc.org-articulo.oa?id=173341774006 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Electronic Journal of Biotechnology 18 (2015) 338–342 Contents lists available at ScienceDirect Electronic Journal of Biotechnology Construction and expression of two-copy engineered yeast of feruloyl esterase Min Zhou 1, Zhiqing Huang 1, Bo Zhou 1, Yanliu Luo, Gang Jia, Guangmang Liu, Hua Zhao, Xiaoling Chen ⁎ Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China a r t i c l e i n f o Article history: Received 13 April 2015 Accepted 30 May 2015 Available online 27 July 2015 Keywords: Aspergillus niger Feruloyl esterase Pichia pastoris a b s t r a c t Background: Aspergillus niger has the ability to secrete feruloyl esterase.
However, for economically viable industrial applications, it is necessary to increase their catalytic activities and-or protein yields to satisfy the increasing needs for feruloyl esterases. Results: The gene AnFaeA that encodes a type A feruloyl esterase was successfully expressed in Pichia pastoris by a two-copy engineered yeast.
After a screen in shaker flask, a one-copy strain GSKFA3 having the highest feruloyl este...





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