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Xiujuan Zheng ; Hongrui Cao ; Linghui Jiang ; Fangqian Liu ; Xinli Sun ;Electronic Journal of Biotechnology 2015, 18 5

Autor: Sique Chen

Fuente: http://www.redalyc.org/


Introducción



Electronic Journal of Biotechnology E-ISSN: 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile Chen, Sique; Zheng, Xiujuan; Cao, Hongrui; Jiang, Linghui; Liu, Fangqian; Sun, Xinli A simple and efficient method for extraction of Taq DNA polymerase Electronic Journal of Biotechnology, vol.
18, núm.
5, 2015, pp.
1-4 Pontificia Universidad Católica de Valparaíso Valparaíso, Chile Available in: http:--www.redalyc.org-articulo.oa?id=173341774005 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative EJBT-00113; No of Pages 4 Electronic Journal of Biotechnology xxx (2015) xxx–xxx Contents lists available at ScienceDirect Electronic Journal of Biotechnology A simple and efficient method for extraction of Taq DNA polymerase Sique Chen, Xiujuan Zheng, Hongrui Cao, Linghui Jiang, Fangqian Liu, Xinli Sun ⁎ Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural & Forestry University, Fuzhou 350002, China a r t i c l e i n f o Article history: Received 17 February 2015 Accepted 27 July 2015 Available online xxxx Keywords: Ethanol precipitation PCR Purification Taq DNA polymerase a b s t r a c t Background: Thermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Plutheros method.
The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time.
Moreover, we found that 30–40% activity of Taq Pol I was lost at the ammonium sulfate precipitation step, and the product contained a small amount of DNA. Results: We provided a novel, simplified and low-cost method to purify the Taq Pol Ι after overproduction of the enzyme in Escherichia col...





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