Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein 5-IAF and Fluorescence DetectionReportar como inadecuado




Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein 5-IAF and Fluorescence Detection - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma. After derivatization of L-ergothioneine with 5-iodoacetamidofluorescein, the separation was carried out by HPLC on an ODS-2 C-18 sperisorb column by using a linear gradient elution and by HPCE on an uncoated fused silica capillary, 50 µm id, and 60 cm length. The methods were validated and found to be linear in the range of 0.3 to 10 µmol-l. The limit of quantification was 0.27 µmol-l for HPCE and 0.15 µmol-l for HPLC. The variations for intra- and inter-assay precision were around 6 RSD%, and the mean recovery accuracy close to 100% 96.11%.



Autor: Salvatore Sotgia , Elisabetta Pisanu, Gianfranco Pintus, Gian Luca Erre, Gerard Aime Pinna, Luca Deiana, Ciriaco Carru, Angelo Zi

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados