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Background-Aim: Isletregeneration in chronic pancreatitis CP is relevant for managing theassociated loss of endocrine function. Because ductal epithelial cells wereearlier demonstrated to differentiate into pancreatic endocrine mass, weevaluated their proliferation and differentiation in chronic pancreatitis.Methods: Pancreatic ducts were obtained from surgically resected pancreata of12 patients with chronic pancreatitis and 15 control subjects. CK19 positiveductal cells were evaluated for their proliferating and differentiatingabilities upon immunostaining with Ki 67 and hormone positivity for insulin andglucagon, apart from monitoring Pdx 1 expression. Results: In comparison to thecontrols, a greater number of proliferating pancreatic ductal epithelial cellsPDECs were observed under conditions of CP. The increase in Pdx1 expressingPDECs 22% and proliferating Pdx1 expressing PDECs 30% was significant P< 0.04. Number of cells expressing insulin-glucagon in the exocrine ductsincreased significantly in CP as compared to controls P < 0.02 and β cell mass adjacent to the ductsincreased by 28%. Conclusion:Enhanced capability of PDECs to proliferate and differentiate into endocrinemass suggests that PDECs form a source of progenitors for cell based therapy inchronic pancreatitis.

KEYWORDS

Chronic Pancreatitis, Ductal Epithelial Cells, Islet Like Clusters, Cell Differentiation

Cite this paper

Kumar, P. , Radhika, G. , Rao, G. , Reddy, D. and Sasikala, M. 2015 Differentiation of Pancreatic Ductal Epithelial Cells into Insulin Like Cell Clusters in Chronic Pancreatitis. Open Journal of Endocrine and Metabolic Diseases, 5, 177-183. doi: 10.4236-ojemd.2015.512022.





Autor: Pondugala Pavan Kumar1, Gaddipati Radhika1, G. Venkat Rao2, D. Nageshwar Reddy2, M. Sasikala1*

Fuente: http://www.scirp.org/



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