Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry FTICR-MS*Reportar como inadecuado




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The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal G-A-column. Glycopeptide from ovalbumin was efficiently enriched by homemade G-A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G-A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.

KEYWORDS

Glycopeptide, LC-ESI, FTICR-MS

Cite this paper

Xin, L. , Hou, L. , Zhou, Y. , Wang, H. and Yu, H. 2015 Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry FTICR-MS*. Advances in Bioscience and Biotechnology, 6, 624-628. doi: 10.4236-abb.2015.69065.





Autor: Ling Xin, Li Hou, Yue Zhou, Huiping Wang, Heming Yu

Fuente: http://www.scirp.org/



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