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In this study, we characterized and quantified vitamin B12 in canned apple snails, escargots, boiled plain using a microbiological assay based on Lactobacillus delbrueckii ATCC 7830. Vitamin B12 contents of canned escargots boiled plain were varied from approximately 0.8 μg-100g weight to approximately 5.5 μg-100g weight mean values, 2.2 μg-100g weight. We identified vitamin B12 compounds from escargots using liquid chromatography-electrospray ionization-tandem mass spectrometry. We found that escargots contained true vitamin B12 and two inactive corrinoids, which were identified as factor IIIm or methoxymensimidazolyl cyanocobamide, and factor S or 2-methylmercaptoadenyl cyanocobamide. These results indicate that canned escargots boiled plain are not good sources of vitamin B12 for humans.

KEYWORDS

Apple Snails, Escargots, Canned Products, Factor S, Factor IIIm, Vitamin B12

Cite this paper

Teng, F. , Tanioka, Y. , Bito, T. , Takenaka, S. , Yabuta, Y. and Watanabe, F. 2015 Occurrence of Biologically Inactive Corrinoid Compounds in Canned Edible Apple Snails Escargots. Food and Nutrition Sciences, 6, 1071-1077. doi: 10.4236-fns.2015.612111.





Autor: Fei Teng1, Yuri Tanioka2, Tomohiro Bito1, Shigeo Takenaka3, Yukinori Yabuta1, Fumio Watanabe1*

Fuente: http://www.scirp.org/



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