Selection of Tolerant Lines to Salinity Derived from Durum Wheat Triticum durum Desf. in Vitro CultureReportar como inadecuado




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The genetic variability is considered as the major principle of plant breeding for durum wheat. This variability can be induced in vitro by selection pressure exerted by stress factors such as salinity in order to regenerate the vitro plantlets tolerant. This study aims in the first step in the regeneration of plantlets tolerant to salinity from mature embryos culture derived from two Tunisian durum wheat varieties: improved Razzek and landrace Jenah Khotifa JK varieties. The tolerance evaluation to salt stress was applied in vitro 100 mmol·l-1 NaCl and was based on various parameters. Our results showed that JK variety was distinguished by a stable response for all parameters tested: average weight of callus 368.1 mg for control and 307 mg under salt stress, callus regenerated percentage 36.6% for control and 35.7% under salt stress and green shoots number-callus 17 for control and 17 under salt stress. This stability of response translates the adaptability of this variety to salinity. In order to fix regenerated JK plantlets in single generation and obtain HDs homozygous stable lines, in vitro gynogenesis technical is tested for this genotype. The Evaluation of gynogenetic capacity focused on about 1200 unfertilized ovaries of JK and was based on its ability to induction, differentiation, development of green shoots, and haploid plantlets regeneration. JK showed good tolerance to salinity and a relatively good response to gynogenesis.

KEYWORDS

Diversity, Mature Embryos Culture, Gynogenesis, Salinity Tolerance, Triticum durum

Cite this paper

Ayed-Slama, O. , Ayed, S. and Slim-Amara, H. 2015 Selection of Tolerant Lines to Salinity Derived from Durum Wheat Triticum durum Desf. in Vitro Culture. Agricultural Sciences, 6, 699-706. doi: 10.4236-as.2015.67067.





Autor: O. Ayed-Slama, S. Ayed, H. Slim-Amara

Fuente: http://www.scirp.org/



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