Bone Formation in a Scaffold Composed of Cylindrical Hydroxyapatite and Tryptophan- or Lysine-Coated Sponge in VivoReportar como inadecuado




Bone Formation in a Scaffold Composed of Cylindrical Hydroxyapatite and Tryptophan- or Lysine-Coated Sponge in Vivo - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Because of the three-dimensional structure of bone or hard tissue such as a tooth, a scaffold is necessary for its regeneration by cellular engineering. Commonly, for in vivo examination, hydroxyapatite HA has been used as such a scaffold. Cylindrical HA with a hollow center, which included a columnar formalin-treated polyvinyl alcohol sponge, was used in this examination as a scaffold. The sponge had been coated with L-tryptophan or L-lysine before insertion into the hollow center of the HA. Rat bone marrow cells rBMCs derived from the femur were seeded in the sponge before insertion into the hollow center of HA. The number of rBMCs seeded in each sponge was 1.5 × 106. These scaffolds were implanted subcutaneously into the backs of Fischer 344 rats for 6 weeks. In the amino-acid-coated sponge in HA, osteogenesis was found histologically. An osteocalcin level of approximately 10 ug was measured in the scaffolds with L-tryptophan-coated formalized poly-vinyl alcohol sponge containing rBMCs, 4 ug on average in the scaffolds with L-lysine-coated sponge containing the cells and about 2 ug in each scaffold with uncoated sponge containing the cells. The structure of the scaffolds used in this study was thought to be suitable for osteogenesis by rBMCs. It was concluded that tryptophan, as a factor for bone formation by stem cells, functioned by promoting cell adhesion and the differentiation of stem cells into osteoblasts.

KEYWORDS

Scaffold, Hydroxyapatite, Bone Marrow Cells, L-Tryptophan, L-Lysine, Poly-Vinyl Formal Sponge

Cite this paper

Yoshikawa, M. , Kakigi, H. , Maeda, H. , Nishikawa, I. , Ikenaga, H. , Inamoto, T. and Tsuji, N. 2015 Bone Formation in a Scaffold Composed of Cylindrical Hydroxyapatite and Tryptophan- or Lysine-Coated Sponge in Vivo. Journal of Biomedical Science and Engineering, 8, 389-398. doi: 10.4236-jbise.2015.86037.





Autor: Masataka Yoshikawa*, Hideyuki Kakigi, Hiroshi Maeda, Ikuo Nishikawa, Hideaki Ikenaga, Takeshi Inamoto, Norimasa Tsuji

Fuente: http://www.scirp.org/



DESCARGAR PDF




Documentos relacionados