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This study investigated the influence of different titanium surfaces on the differentiation of rat osteoblast-like cells osteo-1. Osteo-1 cells were cultured on the following titanium surfaces: 1 pretreated, smooth surface PT; 2 sandblasted and acid etched surface SLA; and 3 sandblasted and acid-etched surface rinsed under nitrogen protection to prevent exposure to air and preserved in isotonic saline solution modSLA. Cell metabolism, total protein content, collagen content and alkaline phosphatase AP activity and the formation of calcified nodules were analyzed. The titanium surface did not influence cell metabolism, total protein content and collagen content. The SLA surface influenced cell differentiation, with the observation of a significant reduction of AP activity and formation of calcified nodules after 21 days compared to the PT surface. No difference was observed between the PT and modSLA surfaces. All titanium surfaces tested permitted the full expression of the osteoblast phenotype by osteo-1 cells, including matrix mineralization.

KEYWORDS

Titanium, Dental Implants, Osteoblasts

Cite this paper

Cirano, F. , Togashi, A. , Marques, M. , Pustiglioni, F. and Lima, L. 2015 The Effects of Different Titanium Surfaces on the Behaviour of Osteoblast-Like Cells. Journal of Biomedical Science and Engineering, 8, 380-388. doi: 10.4236-jbise.2015.86036.





Autor: Fabiano Ribeiro Cirano1, Adriane Yaeko Togashi2*, Márcia Martins Marques3, Francisco Emílio Pustiglioni4, Luiz Antônio Puglies

Fuente: http://www.scirp.org/



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