In Vitro and in Vivo Anti-Inflammatory Effect of a Biotechnologically Modified Borage Seed Extract: Evidence for Lipid Pro-Resolving Mediators’ Implication in the Enhancement of Psoriatic and Atopic Dermatitis LesionsReportar como inadecuado




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Aim: Resolvins, maresins and lipoxins are lipid mediators issued from essentialpolyunsaturated fatty acids which are the first anti-inflammatory and pro-resolvingsignals identified during the resolution phase of inflammation. As borage oil and-orborage seed extracts have shown beneficial action in treatment of atopic dermatitisor eczema in human and canine, we have modified a borage oil component by usingbiotechnology in order to get a compound structurally related to a polyunsaturatedfatty acid, and we have studied its ability to reduce inflammation mediators productionthrough the generation of resolvins, maresins and-or lipoxins. Additionally, wehave demonstrated the potent anti-inflammatory effect of this new compound whichconsists in borage seed oil aminopropanediol amides, through an in vivo study concerning subjects sufferingfrom psoriasis or atopic dermatitis. Study Design-Methods: For the in vitro study, inflammation was inducedin co-cultures of human dendritic cells and normal keratinocytes by the additionof PMA and the calcium ionophore A23187. Ability of our borage seed oil aminopropanediolamides to increase resolvin D2, maresin 1 and lipoxins A4 and B4 synthesis was thenmeasured. Pro-inflammatory cytokines IL-1β,IL-6, IL-8 and PGE2 productions were also quantified. For the in vivo study, 36 subjects suffering frompsoriasis or atopic dermatitis have used twice a day during 30 days, a formulationcontaining borage seed oil aminopropanediol amides. Before the beginning of thestudy and after 30 days’ treatment, the severity of psoriasis and of atopic dermatitiswas evaluated by using the PGA and the SCORAD scoring scales, respectively. Results:Borage seed oil aminopropanediol amides were able to significantly increase theresolvin D2, maresin 1 and lipoxins A4 and B4 synthesis. Concomitantly, they werealso able to significantly inhibit the production of IL-1β, IL-6, IL-8 and PGE2 induced by the PMA and the calcium ionophoreA23187 in the in vitro co-culture modelused. Introduced in formulation, borage seed oil aminopropanediol amides significantlyreduced the clinical manifestations of psoriasis and atopic dermatitis. Conclusion:Our in vitro and in vivo study clearly showed the anti-inflammatory activity of borageseed oil aminopropanediol amides and emphasized the putative role of pro-resolvinglipid mediators in the treatment of atopic dermatitis, psoriasis or other inflammation-inducedskin diseases.

KEYWORDS

Human Skin, Biotechnologically Modified Borage Extract, Interleukins, Inflammation, Psoriasis, Atopic Dermatitis, Resolvins, Maresins, Lipoxins

Cite this paper

Chene, G. , Baillif, V. , Goethem, E. , Branka, J. , Ionescu, T. , Robert, G. and Lefeuvre, L. 2015 In Vitro and in Vivo Anti-Inflammatory Effect of a Biotechnologically Modified Borage Seed Extract: Evidence for Lipid Pro-Resolving Mediators’ Implication in the Enhancement of Psoriatic and Atopic Dermatitis Lesions. Journal of Cosmetics, Dermatological Sciences and Applications, 5, 151-160. doi: 10.4236-jcdsa.2015.52018.





Autor: Gérald Chene1, Vincent Baillif1, Emeline Van Goethem1, Jean-Eric Branka2, Toni Ionescu3, Géraldine Robert3, Luc Lefeuvre3*

Fuente: http://www.scirp.org/



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