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Samplingfor coliform bacterial indicators such as Escherichiacoli E. coli provides auniversally accepted gauge of the microbiologic quality of fresh surface watersworldwide. Protocols for the capture, preservation, and analysis of indicatorbacteria collected from moving waters e.g., rivers, streams, canals, etc.parallel those for collecting bacteria from standing waters e.g., ponds,lakes, and impoundments. Strict depth- and width-integrated rules establishedfor testing moving waters are likely a result of the historical precedence ofour knowledge of bacterial stratification in standing waters. Samplingprotocols for indicator bacteria in freshwater streams recommend capture andretrieval of samples from the mid-water column directed into the current andwithin the deepest portion of the channel to prevent collection of eitherbenthic particles or surface films. Chi-square analyses of multiple stratifiedsamples captured on the same date and time reveal that variability in samplingposition at specified depths within the main stream column or within randomlychosen locations within the main stream channel has no effect p ≥ 0.25 uponsuch indicator bacteria numbers. Additionally, these data are the first to showthat concentrations of the common bacterial indicator, E. coli, are homogeneously distributed throughout both lateral areaand vertical water column within-near a single sampling location of a movingwater body up to 245 cubic feet per second cfs discharge. Moreover, one datapoint bacterial sample appears to represent the overall bacterialconcentration of a small freshwater stream obtained from any single samplinglocation within-near the main channel for a given date and time. These findingssuggest some latitude in sampling strategies for assessing small freshwaterstreams for indicator bacteria such as E.coli for workers in both environmental and public health fields.

KEYWORDS

Indicator Bacteria, Stratification, Water Monitoring, E. coli

Cite this paper

Buckalew, D. , Tuono, A. , Simmons, A. , Lankford, T. and Smith, D. 2015 Homogeneous Distribution of Escherichia coli Measured within the Vertical Water Column of Small, Freshwater Streams. Journal of Water Resource and Protection, 7, 410-421. doi: 10.4236-jwarp.2015.75033.





Autor: David. W. Buckalew1*, Albert. J. Tuono1, Amanda. K. Simmons1, Travis. W. Lankford1, Donald. H. Smith2

Fuente: http://www.scirp.org/



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