Novel Strategies for Enhanced Removal of Persistent Bacillus anthracis Surrogates and Clostridium difficile Spores from SkinReportar como inadecuado




Novel Strategies for Enhanced Removal of Persistent Bacillus anthracis Surrogates and Clostridium difficile Spores from Skin - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Removing spores of Clostridium difficile and Bacillus anthracis from skin is challenging because they are resistant to commonly used antimicrobials and soap and water washing provides only modest efficacy. We hypothesized that hygiene interventions incorporating a sporicidal electrochemically generated hypochlorous acid solution Vashe® would reduce the burden of spores on skin.

Methods

Hands of volunteers were inoculated with non-toxigenic C. difficile spores or B. anthracis spore surrogates to assess the effectiveness of Vashe solution for reducing spores on skin. Reduction in spores was compared for Vashe hygiene interventions versus soap and water control. To determine the effectiveness of Vashe solution for removal of C. difficile spores from the skin of patients with C. difficile infection CDI, reductions in levels of spores on skin were compared for soap and water versus Vashe bed baths.

Results

Spore removal from hands was enhanced with Vashe soak >2.5 log10 reduction versus soap and water wash or soak ~2.0 log10 reduction; P <0.05 and Vashe wipes versus alcohol wipes P <0.01. A combined approach of soap and water wash followed by soaking in Vashe removed >3.5 log10 spores from hands P <0.01 compared to washing or soaking alone. Bed baths using soap and water N =26 patients did not reduce the percentage of positive skin cultures for CDI patients 64% before versus 57% after bathing; P =0.5, whereas bathing with Vashe solution N =21 patients significantly reduced skin contamination 54% before versus 8% after bathing; P =0.0001. Vashe was well-tolerated with no evidence of adverse effects on skin.

Conclusions

Vashe was safe and effective for reducing the burden of B. anthracis surrogates and C. difficile spores on hands. Bed baths with Vashe were effective for reducing C. difficile on skin. These findings suggest a novel strategy to reduce the burden of spores on skin.



Autor: Michelle M. Nerandzic , Elze Rackaityte, Lucy A. Jury, Kevin Eckart, Curtis J. Donskey

Fuente: http://plos.srce.hr/



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