Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte DifferentiationReportar como inadecuado




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The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide CER, comprising an ultra long-chain ULC fatty acid FA of ≥26 carbons ULC CER, plays an essential role in barrier formation. ULC acyl-CoAs, produced by the FA elongase ELOVL4, are converted to ULC CERs by the CER synthase CERS3. In the presented study, we observed that ELOVL4 and CERS3 mRNAs increased during keratinocyte differentiation in vivo and in vitro. We also determined that peroxisome proliferator-activated receptor β-δ is involved in the up-regulation of the mRNAs. Knockdown of CERS3 caused a reduction in the elongase activities toward ULC acyl-CoAs, suggesting that CERS3 positively regulates ULCFA. Thus, we reveal that the two key players in ULC CER production in epidermis, CERS3 and ELOVL4, are coordinately regulated at both the transcriptional and enzymatic levels.



Autor: Yukiko Mizutani, Hui Sun, Yusuke Ohno, Takayuki Sassa, Takeshi Wakashima, Mari Obara, Kohei Yuyama, Akio Kihara , Yasuyuki Igaras

Fuente: http://plos.srce.hr/



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