Towards a More Precise Serological Diagnosis of Human Tegumentary Leishmaniasis Using Leishmania Recombinant ProteinsReportar como inadecuado




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Background

Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure.

Methodology-Principal Findings

Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis TL against different L. infantum-chagasi-derived recombinant proteins rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11. Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics ROC curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas- disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA.

Conclusion

Our results raise possibility of using rHSP70 for the serodiagnosis of TL



Autor: Ana Paula Souza, Manuel Soto, Jackson M. L. Costa, Viviane S. Boaventura, Camila I. de Oliveira, Juqueline R. Cristal, Manoel Bar

Fuente: http://plos.srce.hr/



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