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Objective: The aim of thisstudy was correlation proliferative activity, markers express stem cells, andlipid peroxides of undifferentiated stem cells of human adult dental follicleDF following culture. Methods: For this study, we used 8 samples from DF ofimpacted third molars to maintain culture conditions and evaluated the growthcurve, cell viability, production of lipid peroxidation, cell cycle phases, andproliferative index during 25 days of culture. Results: Cells after cultureshowed characteristics of fibroblast-like type following 25th day of culture.The results of lipid peroxidation showed that stem cells in culture produce 13nmoles-ml malondialdehyde at the start of culture, increasing until the 12thday and then began a decline that lasted until the 25th day. We revealed thatDFSCs presented a significantly higher percentage of cells in S + G2-M phasesby the 15th day of culture compared with cells at the start of culture. Cellsurface markers revealed that cell lines were negative for HLA-DR and positivefor CD90, CD44, and CD105. The expression of p21 protein, involved in theregulation of the cell cycle, showed a significant increase from the 15th to25th day of culture. Results of cell division rates show a significant increasebetween the 6th and 15th day of culture. Conclusions: We conclude that theculture remained stable during the 25 days of culture, presenting the markersof stem cells and markers of control, progression, and cell proliferation thatthere was an increased production of lipid peroxides between the 6th and 12thdays; this increase is related to the increased numbers of cells that alsooccurs during this period. Then, there is a significantly decline in theproduction of lipid peroxides and the number of cells, which is accompanied byan increase in cell unviability.

KEYWORDS

Follicle Dental Stem Cells, Lipid Peroxidation, Cell Cycle, Proliferation

Cite this paper

Carvalho, L. , Araujo, A. , Silva, M. , Laiso, R. and Maria, D. 2014 Response Proliferative Capacity of Undifferentiated Stem Cells of Obtained Human Adult Dental Follicle. Stem Cell Discovery, 4, 125-137. doi: 10.4236-scd.2014.44013.





Autor: Larissa Kim Higashi de Carvalho1,2, Aline Vieira Pinheiro de Araujo1,2, Manuela Garcia Laveli da Silva1,2, Rosa Andréa Nogueira

Fuente: http://www.scirp.org/



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