Effect of Propofol in the Immature Rat Brain on Short- and Long-Term Neurodevelopmental OutcomeReportar como inadecuado




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Background

Propofol is commonly used as sedative in newborns and children. Recent experimental studies led to contradictory results, revealing neurodegenerative or neuroprotective properties of propofol on the developing brain. We investigated neurodevelopmental short- and long-term effects of neonatal propofol treatment.

Methods

6-day-old Wistar rats P6, randomised in two groups, received repeated intraperitoneal injections 0, 90, 180 min of 30 mg-kg propofol or normal saline and sacrificed 6, 12 and 24 hrs following the first injection. Cortical and thalamic areas were analysed by Western blot and quantitative real-time PCR qRT-PCR for expression of apoptotic and neurotrophin-dependent signalling pathways. Long-term effects were assessed by Open-field and Novel-Object-Recognition at P30 and P120.

Results

Western blot analyses revealed a transient increase of activated caspase-3 in cortical, and a reduction of active mitogen-activated protein kinases ERK1-2, AKT in cortical and thalamic areas. qRT-PCR analyses showed a down-regulation of neurotrophic factors BDNF, NGF, NT-3 in cortical and thalamic regions. Minor impairment in locomotive activity was observed in propofol treated adolescent animals at P30. Memory or anxiety were not impaired at any time point.

Conclusion

Exposing the neonatal rat brain to propofol induces acute neurotrophic imbalance and neuroapoptosis in a region- and time-specific manner and minor behavioural changes in adolescent animals.



Autor: Tanja Karen , Gerald W. Schlager , Ivo Bendix, Marco Sifringer, Ralf Herrmann, Christos Pantazis, David Enot, Matthias Keller, Th

Fuente: http://plos.srce.hr/



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