Surfactant Protein SP-A Suppresses Preterm Delivery and Inflammation via TLR2Reportar como inadecuado




Surfactant Protein SP-A Suppresses Preterm Delivery and Inflammation via TLR2 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Toll like receptors TLRs are pattern-recognition molecules that initiate the innate immune response to pathogens. Pulmonary surfactant protein SP-A is an endogenously produced ligand for TLR2 and TLR4. SP-A has been proposed as a fetally produced signal for the onset of parturition in the mouse. We examined the effect of interactions between SP-A and the pathogenic TLR agonists lipopolysaccharide LPS, peptidoglycan PGN and polyinosinic:cytidylic acid polyI:C ligands for TLR4, TLR2 and TLR3, respectively on the expression of inflammatory mediators and preterm delivery. Three types of mouse macrophages the cell line RAW 264.7, and fresh amniotic fluid and peritoneal macrophages, including macrophages from TLR4 and TLR2 knockout mice were treated for up to 7 hours with pathogenic TLR agonists with or without SP-A. SP-A alone had no effect upon inflammatory mediators in mouse macrophages and did not independently induce preterm labor. SP-A significantly suppressed TLR ligand-induced expression of inflammatory mediators interleukin IL-1β, tumor necrosis factor TNF-α and the chemokine CCL5 via a TLR2 dependent mechanism. In a mouse inflammation-induced preterm delivery model, intrauterine administration of SP-A significantly inhibited preterm delivery, suppressed the expression of proinflammatory mediators and enhanced the expression of the CXCL1 and anti-inflammatory mediator IL-10. We conclude that SP-A acts via TLR2 to suppress TLR ligand-induced preterm delivery and inflammatory responses.



Autor: Varkha Agrawal , Keith Smart, Tamas Jilling, Emmet Hirsch

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados