Singlet Oxygen Induced Products of Linoleates, 10- and 12-Z,E-Hydroxyoctadecadienoic Acids HODE, Can Be Potential Biomarkers for Early Detection of Type 2 DiabetesReportar como inadecuado




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Current diagnostic tests such as glycemic indicators have limitations for early detection of impaired glucose tolerance IGT, which leads to diabetes. Oxidative stress induced by various oxidants in a random and destructive manner is considered to play an important role in the pathophysiology of a number of human disorders and diseases such as impaired glucose tolerance. We have developed an improved method for the measurement of in vivo lipid peroxidation, where the presence of 8-iso-prostaglandin F2α 8-iso-PGF2α, hydroxyoctadecadienoic acids HODEs, hydroxyeicosatetraenoic acids HETEs, and 7-hydroxycholesterol 7-OHCh, as well as their parent molecules, linoleic acid LA and cholesterol Ch, was determined by performing LC-MS-MS for 8-iso-PGF2α, HODE, and HETE and GC-MS for 7-OHCh, LA, and Ch after reduction with triphenyl phosphine and saponification by potassium hydroxide. We then applied this method to volunteers n = 57, including normal type n = 43 -high-normal- fasting plasma glucose, 100–109 mg-dL, n = 7, pre-diabetic type IGT, n = 5, and diabetic type n = 2 subjects who are diagnosed by performing oral glucose tolerance tests OGTTs. Several biomarkers in plasma, such as insulin, leptin, adiponectin, interleukin-6, tumor necrosis factor-α, high sensitivity-C-reactive protein, HbA1c, and glucose levels were measured during OGTT. We found that the fasting levels of 10- and 12-Z,E- HODE-LA increased significantly with increasing levels of HbA1c and glucose during OGTT and with insulin secretion and resistance index. In conclusion, 10- and 12-Z,E-HODE may be prominent biomarkers for the early detection of IGT and -high-normal- type without OGTT.



Autor: Aya Umeno, Mototada Shichiri , Noriko Ishida, Yoshiko Hashimoto, Kaori Abe, Masatoshi Kataoka, Kohzoh Yoshino, Yoshihisa Hagihara

Fuente: http://plos.srce.hr/



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